TEST PROCEDURE 1. Remove the Test Device from the protective pouch and place on a flat surface. Label the Test Device with the subject I.D. or control identification. 2. Gently mix the sample by inverting. 3. Using the Transfer Pipette provided, transfer 1 drop of sample (whole blood, serum, or plasma) in to the sample well. Wait for the sample to be absorbed (3-5 seconds). 4. Holding the Chase Buffer Bottle vertically, promptly add 2 drops of the Chase Buffer to the sample well. 5. Read the results within 10 minutes. High positives may appear as soon as 1 minute, and low positive results may take up to 10 minutes to appear. Do not read results after 15 minutes. Colored lines which appear after 15 minutes are not diagnostic and should be ignored. INTERPRETATION OF TEST RESULTS Positive Results The test is positive if two colored lines appear. One colored line will appear at the Test line (T) area and other at the Control line (C) area. Any intensity of the Test line (T) area should be considered positive. Colored lines may be lighter or darker than each other. Negative Results The test is negative if only one line appears at the Control line (C) area. Invalid Results The test is invalid if no colored line appears at the Control line (C) area even if a colored line appears in the Test line (T) area. If no colored line appears at the Control line (C) within 10 minutes, add an additional drop of the sample and wait for 5 minutes. If a colored line still does not appear in the C area, the test is invalid and should be repeated. Colored lines which appear after 15 minutes are not diagnostic and should be ignored.

• Important: Do not remove device from the pouch until ready for use. • For veterinary use only. • Do not use components after expiration date. • Device must be used as soon as possible after removing from pouch. • The device should be in a horizontal position on a flat surface while the test is performed. • Use a separate transfer pipette for each test. • All wastes should be properly decontaminated prior to disposal. • The buffer reagent is not interchangeable from serial to serial. • Contains Proclin™ 300 and sodium azide as preservatives.